A new look towards BAC-based array CGH through a comprehensive comparison with oligo-based array CGH
نویسندگان
چکیده
منابع مشابه
Model-based clustering of array CGH data
MOTIVATION Analysis of array comparative genomic hybridization (aCGH) data for recurrent DNA copy number alterations from a cohort of patients can yield distinct sets of molecular signatures or profiles. This can be due to the presence of heterogeneous cancer subtypes within a supposedly homogeneous population. RESULTS We propose a novel statistical method for automatically detecting such sub...
متن کاملComprehensive characterization of genomic aberrations in gangliogliomas by CGH, array-based CGH and interphase FISH.
Gangliogliomas are generally benign neuroepithelial tumors composed of dysplastic neuronal and neoplastic glial elements. We screened 61 gangliogliomas [World Health Organization (WHO) grade I] for genomic alterations by chromosomal and array-based comparative genomic hybridization (CGH). Aberrations were detected in 66% of gangliogliomas (mean +/- SEM = 2.5 +/- 0.5 alterations/tumor). Frequent...
متن کاملHuman and mouse oligonucleotide-based array CGH
Array-based comparative genomic hybridization is a high resolution method for measuring chromosomal copy number changes. Here we present a validated protocol using in-house spotted oligonucleotide libraries for array comparative genomic hybridization (CGH). This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multi-copy amplifications as well ...
متن کاملEvaluating array-CGH based clustering of tumors
DNA preparation to cancer profiles •Many of the changes in the genome associated to cancer can be seen by making gene copy number measurements. Increased copy numbers of genes are associated with oncogenes and decreased copy numbers are associated with tumor suppressor genes. •Array-based Comparative Genomic Hybridization (arrayCGH), introduced in the late nineties, allows efficient characteriz...
متن کاملBAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH)
The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is used in human genetics and oncology, with great promise for clinical application. Until recently primarily PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted as elements on the array. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Genomics
سال: 2007
ISSN: 1471-2164
DOI: 10.1186/1471-2164-8-84